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crispri vector krab dcas9 mecp2  (Addgene inc)


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    Structured Review

    Addgene inc crispri vector krab dcas9 mecp2
    Crispri Vector Krab Dcas9 Mecp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+krab+mecp2+vector/dCas9-KRAB-MeCP2+(Plasmid+%23110821)/bio_rxiv__64898__2026__02__11__705362-218-10-13
    Average 94 stars, based on 95 article reviews
    crispri vector krab dcas9 mecp2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Construct:

    Article Title: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 Savell et al., , , ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector [a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 Yeo et al., ). ..

    Article Title: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 )). ..

    Expressing:

    Article Title: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 Savell et al., , , ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector [a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 Yeo et al., ). ..

    Article Title: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 )). ..

    Cloning:

    Article Title: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 Savell et al., , , ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector [a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 Yeo et al., ). ..

    Article Title: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 )). ..

    Plasmid Preparation:

    Article Title: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 Savell et al., , , ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector [a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 Yeo et al., ). ..

    Article Title: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 )). ..

    Polymerase Chain Reaction:

    Article Title: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 Savell et al., , , ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector [a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 Yeo et al., ). ..

    Article Title: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 )). ..

    Amplification:

    Article Title: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 Savell et al., , , ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector [a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 Yeo et al., ). ..

    Article Title: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
    Article Snippet: .. To generate a lentivirus-compatible dCas9-KRAB-MeCP2 construct capable of robust neuronal expression, a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #114196 ), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #110821 )). ..



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